Table 2.
Purification of recombinant human CES1 from E. coli.
| Construct | Purification step | Total protein (mg) | Specific activity (μmol/min/mg) | Yield (%) | Refolding yield (%) |
|---|---|---|---|---|---|
| WT | Solubilized in 8 M urea | 27.1 | – | 100 | 9.0 |
| Cobalt resin column | 13.4 | – | 49.4 | ||
| Refolding | 1.2 | 73 | 4.4 | ||
| S76N | Solubilized in 8 M urea | 31.2 | – | 100 | 10.2 |
| Cobalt resin column | 16.6 | – | 53.2 | ||
| Refolding | 1.7 | 128 | 5.4 | ||
| D204E | Solubilized in 8 M urea | 25.7 | – | 100 | 19.1 |
| Cobalt resin column | 11.6 | – | 45.1 | ||
| Refolding | 2.21 | 193 | 8.6 | ||
| A270S | Solubilized in 8 M urea | 29.3 | – | 100 | 11.9 |
| Cobalt resin column | 12.6 | – | 43.0 | ||
| Refolding | 1.5 | 109 | 5.1 | ||