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Kinetic parameters of CES1, WT and mutants. Data are expressed as the mean ± standard deviation (S.D.).
| Construct | KM (μM) | Vmax (μmol/min/mg) | kcat (s−1) |
|---|---|---|---|
| Wild type | 579 ± 79 | 73 ± 4 | 7.6 ± 0.4 |
| S76N | 389 ± 81 | 128 ± 10 | 13 ± 1 |
| D204E | 417 ± 98 | 193 ± 15 | 20 ± 2 |
| A270S | 263 ± 53 | 109 ± 6 | 11 ± 0.6 |
