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. Author manuscript; available in PMC: 2015 Jan 14.
Published in final edited form as: J Mol Biol. 2008 Oct 11;384(4):954–966. doi: 10.1016/j.jmb.2008.09.083

Scheme 1.

Scheme 1

Two-step formation of LC8-ligand complexes, where X is the ligand and the dissociation constants are defined as K1d = [LC8][X]/[LC8 − X] and K2d = [LC8 − X][X]/[X − LC8 − X]. The binding sites and ligands are identical, so [LC8−X]=[X−LC8].