TABLE 1.

Oligonucleotide primers used in this study

Restriction enzyme sites are underlined.

Oligonucleotide	Sequence (5′–3′)	Aim
EGCrP-05607S-NotI	ATGGCGGCCGCATGCCTCCTCCACCAGAAGTCTCTCCTGTCA	Expression
EGCrP-05607AS-XhoI	ATGCTCGCGAGCAATAACGCATTCAGGACATC
13CN005607seq-U	GACGGCAAGAACATTCAAGACT	Sequencing
14CN005607seq-D	CGTGAGGGTAGCTGAGGAGTT
CN05607N-U	ATGCCTCCTCCACCAGAAGTCT	Gene disruption
CN05607N-AP-D	AATAGCGAGTCCATCGTCGAGATTCAAAGGT
ActinP-U	ACTCGCTATTGTCCAGGCTGC
Act-Nat-Down	CTTCTTCTTCCATAGACATGTTGGGCGAG
Actin-HygroR-D	CTCGACAGACGTCGCGGTGAGCATAGACATGTTGGGCGAGT
Nat-Up	ATGGAAGAAGAAGTCACTCTTGACGACACGGCTTACC
Hygro-U	CTCACCGCGACGTCTGTCGAG
NSL-2	AAGGTGTTCCCCGACGACGAATCG
NSR-2	AACTCCGTCGCGAGCCCCATCAAC
HSL	GGATGCCTCCGCTCGAAGTA
HSR	CGTTGCAAGACCTGCCTGAA
Nat-Ttrp-Down	TAACCCCTTACCGCCTTGGGGCAGGGCATGCTCA
Hygro-TrpT-D	GTATATATACACCCTCTAAGGAAAACTATTCCTTTGCCCTCGGACG
Ttrp-Up	AAGGCGGTAAGGGGTTAATTTTCCTTAGAGGGTG
Trp-U(hyg)	TTTTCCTTAGAGGGTGTATATATAC
TrpT-CN05607C-D	GGATCGATACAGATGAGGGGTGCGACAGAAGAGA
CN05607C-U	ACCCCTCATCTGTATCGATCC
CN05607C-D	CTAAGCAATAACGCATTCAGG
E2-5SENSE	CGGTGACTTAATTAGAACGCTG	Southern blot
E2-5ANTISENSE	CCATCGTCGAGATTCAAAGGT
3Probe-S	TCCGAAAAGAGTCCACAGAG
3Probe-A	TTGTTCCTGCCCTGGTTG