TABLE 3.

¹³C and ¹H NMR spectral data of EG and ergosterol

NMR chemical shifts of standard ergosterol were assigned by MQ-COSY, TOCSY, HSQC, and HMBC experimental data, which measured at the same condition of EG.

13C NMR data				1H NMR data
Carbon	EG	Ergosterol	Multiplicity	Proton	EG	Ergosterol
1	39.0	39.0	CH2	1α	1.32	1.30
				1β	1.93	1.90
2	30.5	32.0	CH2	2α	2.00	1.86
				2β	1.67	1.50
3	78.2	70.4	CH	3	3.72 (m)	3.54 (m)
4	37.8	40.9	CH2	4α	2.60 (brd, 14.7)	2.44
				4β	2.33 (brt, 13.0)	2.27
5	140.1	140.6	C=
6	120.5	120.1	CH=	6	5.57 (brs)	5.55
7	116.9	116.9	CH=	7	5.38 (brs)	5.38
8	141.8	141.6	C=
9	46.9	46.9	CH	9	1.99	1.97
10	37.8	37.6	C
11	21.6	21.7	CH2	11α	1.74	1.74
				11β	1.62	1.62
12	39.7	39.7	CH2	12α	1.30	1.28
				12β	2.09	2.09
13	43.4	43.4	C
14	55.1	55.1	CH	14	1.92	1.90
15	23.5	23.5	CH2	15α	1.69	1.67
				15β	1.32	1.38
16	28.8	28.8	CH2	16α	1.79	1.78
				16β	1.36	1.34
17	56.4	56.4	CH	17	1.30	1.28
18	12.4	12.4	CH3	18	0.65 (3H, s)	0.65
19	16.5	16.5	CH3	19	0.96 (3H, s)	0.95
20	41.1	41.0	CH	20	2.06	2.06
21	21.4	21.4	CH3	21	1.05 (3H, d, 6.7)	1.05
22	136.3	136.2	CH=	22	5.20 (dd, 7.8, 15.3)	5.19
23	132.6	132.6	CH=	23	5.24 (dd, 7.3, 15.3)	5.24
24	43.6	43.5	CH	24	1.87	1.86
25	33.7	33.7	CH	25	1.48	1.48
26	19.9	19.9	CH3	26	0.84 (3H, d, 6.9)	0.84
27	20.2	20.2	CH3	27	0.85 (3H, d, 6.9)	0.85
28	17.9	18.0	CH3	28	0.93 (3H, d, 6.7)	0.93
β-Glc
C-1′	101.9		CH	H-1′	4.43 (d, 7.8)
C-2′	74.3		CH	H-2′	3.22 (t, 8.4
C-3′	77.3		CH	H-3′	3.41 (t, 8.8)
C-4′	80.0		CH	H-4′	3.38 (t, 9.0)
C-5′	76.9		CH	H-5′	3.30 (m)
C-6′	62.3		CH2	H-6a′	3.73 (dd, 5.2, 11.9)
				H-6b′	3.87 (dd, 2.7, 11.9)