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Assembly of Syn6301RbcL Rubisco enzymes using heterologous red-type small subunits or green/red hybrid RbcS. A, assembly of Syn6301RbcL with Syn6301RbcS or RbcS subunits from R. sphaeroides, G. sulfuraria, or P. tricornutum or Syn6301RbcSEF with or without AnaCARbcX as indicated. Reconstitution reactions were performed and analyzed as in Fig. 4B. B, Rubisco activities in reactions as described in A. Error bars indicate S.D. of at least three independent experiments.
