FIGURE 3.

Different subcellular localizations of cTPC3 and rTPC3 when stably expressed in HEK293 cells. Stable cell lines were made in HEK293 cells using mCherry-tagged (red) cTPC3 (A) or rTPC3 (B) and GFP-tagged organelle markers (green). The upper panels of each image show a GFP-labeled organelle (left) and mCherry-labeled TPC3 (right). The lower panels show a merged image (colocalization in yellow; left) and a bright-field image (right). Scale bars = 10 μm. Pearson coefficients for cells exemplified in the images are shown on the right. Data are means ± S.E. of three cells for each pair. TfR, transferrin receptor.