FIGURE 12.

The neddylation of SCF stimulates the cross-linking of Cdc34 to Cul1. A, Cul1 Western blot of reactions between either Cdc34 1C or Cdc34 2C proteins that had been activated with BMOE and neddylated K679C Cul1-Rbx1. B, graph showing the fraction of either neddylated (black bars) or unneddylated (gray bars) K679C Cul1 subunits that cross-linked to either Cdc34 1C or Cdc34 2C proteins. Error bars, S.E. of measurement from duplicate data points. C and D, same as A and B, except the Cdc34 1C and 2C proteins had been activated with DPS. Note that K679C Cul1 was chosen for these experiments because Lys-679 is centrally located in the basic canyon region.