FIGURE 4.

Dbf4-Cdc7 phosphorylation of Mcm2 is required for GINS-Mcm2-7 interaction during S phase. A, cells from Fig. 2 (C and D) incubated at 37 °C in the presence of 0.15% galactose were arrested with α-factor and released into medium lacking α-factor for the time points indicated. Left panel, whole cell extracts were probed for antibodies directed against Mcm2, Cdc45, Psf2 (a component of GINS), or Sld3. Right panel, co-IP. Unfixed cells were immunoprecipitated with antibodies directed against Mcm2 followed by Western analysis with antibodies directed against Mcm2, Cdc45, Psf2, or Sld3. B, Similar to A, except the indicated cell strains harbored the mcm5-bob1 mutation.