FIGURE 2.
Tps1 mutants fail to produce trehalose and show lethality at the late pupal stage. A, schematic representation of the Tps1 locus and molecular nature of the mutants. Protein-coding regions and untranslated regions are represented by black boxes and white boxes, respectively. The P-element insertion sites are marked with inverted triangles. B, Tps1lacW homozygous mutants are lethal at the pharate adult stage. APF, after puparium formation. C, Tps1 mutants reduce trehalose levels at the late third-instar larval stage. D, trehalose levels in the hemolymph were analyzed in late third-instar larvae of the indicated genotypes. E, Tps1 expression was analyzed by qRT-PCR in mid third-instar larvae. F, Tps1MIC is a hypomorphic allele. The percentage of adult flies was determined by the ratio to flies with a balancer chromosome in each vial. All values are means ± S.D. (n > 6 (C, D, and F) or n = 4 (E)).