TABLE 1.
Analysis of canonical pathways associated with each MEF2 isoform
Each preferentially sensitive MEF2 gene set, including the 21 commonly dysregulated genes, were analyzed using Ingenuity Pathway Analysis software. The top five most significantly regulated canonical pathways are provided. These pathways were analyzed for statistically significant association with each unique MEF2-sensitive gene set, and all were found to be significant (p < 0.05) with the exception of the MEF2D-associated canonical pathways, likely because of the small number of MEF2D-sensitive genes dysregulated in the expression analysis. Also provided is the amount of genes dysregulated in each MEF2 factor knockdown in relation to the accepted number of genes associated with each canonical pathway (ratio).
| Canonical pathway | p value | Ratio |
|---|---|---|
| MEF2A | ||
| Molecular mechanisms of cancer | 2.40E-08 | 92/387 (0.238) |
| Calcium signaling | 2.26E-07 | 55/217 (0.253) |
| Germ cell-Sertoli cell junction signaling | 6.20E-06 | 46/169 (0.272) |
| Actin cytoskeletal signaling | 1.63E-05 | 57/242 (0.236) |
| Signaling by Rho family GTPases | 1.96E-05 | 60/262 (0.229) |
| MEF2B | ||
| Hepatic fibrosis/hepatic stellate cell activation | 1.31E-03 | 5/155 (0.032) |
| Ovarian cancer signaling | 7.57E-03 | 4/152 (0.026) |
| Human embryonic stem cell pluripotency | 7.77E-03 | 4/161 (0.025) |
| Ceramide signaling | 1.13E-02 | 3/91 (0.033) |
| Apoptosis signaling | 1.47E-02 | 3/100 (0.03) |
| MEF2C | ||
| Cell cycle: G1/S checkpoint regulation | 3.14E-03 | 3/68 (0.044) |
| Eicosanoid signaling | 3.14E-03 | 3/85 (0.035) |
| Molecular mechanisms of cancer | 5.49E-03 | 6/387 (0.016) |
| Estrogen-mediated S phase entry | 5.48E-03 | 2/28 (0.071) |
| Sulfate activation for sulfonation | 9.42E-03 | 1/8 (0.125) |
| MEF2D | ||
| Role of JAK2 in hormone-like cytokine signaling | 4.03E-02 | 1/37 (0.027) |
| Hypoxia signaling in the cardiovascular system | 7.69E-02 | 1/68 (0.015) |
| RhoA signaling | 1.32E-01 | 1/122 (0.008) |
| Artherosclerosis signaling | 1.37E-01 | 1/138 (0.007) |
| AMPK signaling | 1.50E-01 | 1/180 |
| All MEF2 | ||
| Regulation of cellular mechanics by calpain protease | 3.20E-05 | 3/73 (0.041) |
| nNOS signaling in neurons | 1.20E-03 | 2/52 (0.038) |
| Integrin signaling | 1.24E-03 | 3/208 (0.014) |
| Amyloid processing | 1.41E-03 | 2/61 (0.033) |
| FAK signaling | 4.05E-03 | 2/106 (0.019) |