FIGURE 1.

FBGCs fail to resorb bone. A and B, osteoclast and FBGC common progenitor cells were cultured in the presence of M-CSF plus RANKL (for osteoclasts) or GM-CSF plus IL-4 (for FBGCs), and cells were subjected to TRAP and May-Grünwald Giemsa staining (bar = 100 μm) (A), a real time PCR assay for Trap expression relative to Gapdh (B), or May-Grünwald Giemsa staining and a bone resorption assay on dentine slices (bar, 100 μm) (C). Data represent means ± S.D of Trap/Gapdh levels (***, p < 0.001; n = 3). Resorbing lacunae were visualized by toluidine blue staining (C, left panel), and the relative area resorbed was quantified. Data represent means ± S.D of the resorbed area in FBGC relative to osteoclast samples (***, p < 0.001; n = 3) (C, right panel). Shown are representative data of at least three independent experiments.