FIGURE 2.

Effects of 30 and 100 nm DOPC liposomes on amyloid fibrillation of Aβ-(1–40) at 37 °C without shaking. A and D, kinetics monitored by ThT fluorescence in the absence and presence of 30 nm (A) or 100 nm (D) DOPC liposomes at various concentrations at 37 °C. The concentrations of the liposomes are described in each figure. Amyloid fibrillation at the respective liposome concentrations was monitored at three wells, and the representative kinetics is shown. The insets show the average lag times. B, C, E, and F, dependences of the relative lag time (B and E) and maximal ThT amplitude (C and F) on the 30-nm (B and C) or 100-nm (E and F) DOPC liposome concentrations. Dotted lines are the values obtained in the absence of liposomes. The error bars and gray zones indicate the S.D. among three experiments.