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. 2014 Nov 21;290(2):841–850. doi: 10.1074/jbc.M114.607796

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FIGURE 2. — Analysis of phospho-oligosaccharides purified from glycogen and amylopectin by HPAEC. Oligosaccharides were separated by HPAEC with PAD using a PA200 column as detailed under “ Experimental Procedures.” For phospho-oligosaccharides, 2.5 nmol based on phosphate content were analyzed. A, polyglucose standards (0.25 nmol) from glucose (G1) up to maltooctaose (G8). B, phospho-oligosaccharides purified from rabbit muscle glycogen prepared by the TCA protocol. C, phospho-oligosaccharides purified from rabbit muscle glycogen prepared by the KOH protocol. D, phospho-oligosaccharides purified from amylopectin. nC, nanoCoulomb.