TABLE 1.
Proton, carbon, and phosphorus chemical shifts (in ppm) of phosphorylated amylopectin and glycogen oligosaccharides
| Residue | 1 | 2 | 3 | 4 | 5 | 6 | ||
|---|---|---|---|---|---|---|---|---|
| aa | t-α-Glc-2-P- (1→) | 1H | 5.67 | 3.98 | 3.76 | 3.49 | 3.70 | 3.83/3.77 |
| 13C | 100.0 | 77.3 | 74.7 | 71.8 | 75.3 | 63.3 | ||
| 31P | 1.12 | |||||||
| b | t-α-Glc- (1→4) | 1H | 5.60 | 3.57 | 3.66 | 3.43 | 3.66 | 3.83/3.77 |
| 13C | 101.1 | 74.0 | 75.4 | 72.1 | 75.4 | 63.3 | ||
| cb | t-α-Glc- (1→4-Glc-6-P) | 1H | 5.48 | 3.56 | 3.70 | 3.35 | NDc | 3.92/3.80 |
| 13C | 101.6 | 74.1 | 75.4 | 72.4 | ND | 63.5 | ||
| d | 4-α-Glc- (1→4) | 1H | 5.39 | 3.59 | 3.97 | 3.64 | 3.84 | 3.83/3.77 |
| 13C | 102.2 | 74.1 | 76.0 | 79.7 | 73.8 | 63.3 | ||
| e | t-α-Glc-6-P | 1H | 5.38 | 3.61 | 3.68 | 3.51 | 3.81 | 4.05/4.05 |
| 13C | 102.4 | 74.1 | 75.4 | 71.5 | 74.5 | 66.2 | ||
| 31P | 2.07 | |||||||
| f | 4-α-Glc-6-P | 1H | 5.37 | 3.63 | ND | 3.71 | 3.98 | 4.12/4.03 |
| 13C | 102.2 | 74.1 | ND | 78.1 | 73.0 | 66.6 | ||
| 31P | 1.02 | |||||||
| g | 4-α-Glc-3-P | 1H | 5.35 | 3.75 | 4.46 | 3.81 | 3.87 | ND |
| 13C | 102.4 | 74.6 | 79.9 | 76.4 | 73.8 | ND | ||
| 31P | 2.81 | |||||||
| h | 4-α-Glcred | 1H | 5.21 | 3.56 | 3.95 | 3.64 | ND | ND |
| 13C | 94.5 | 74.0 | 76.0 | 79.7 | ND | ND | ||
| i | 4-β-Glcred | 1H | 4.64 | 3.27 | 3.75 | 3.64 | ND | ND |
| 13C | 98.5 | 76.6 | 78.8 | 79.7 | ND | ND | ||
| ja | 4,6-α-Glcred | 1H | 5.22 | 3.55 | 3.96 | 3.64 | 3.93 | 3.98/3.83 |
| 13C | 94.6 | 74.2 | 76.0 | 79.7 | 72.7 | 69.7 | ||
| ka | t-α-Glc- (1→6) | 1H | 4.99 | 3.54 | 3.74 | 3.44 | 3.86 | ND |
| 13C | 101.5 | 74.7 | 74.2 | 72.1 | 73.8 | ND | ||
| la | 4-α-Glc- (1→6) | 1H | 4.95 | 3.59 | 4.01 | 3.63 | 3.85 | ND |
| 13C | 100.7 | 74.1 | 73.0 | 80.6 | 74.0 | ND | ||
| ma | 4,6-β-Glcred | 1H | 4.65 | 3.25 | 3.77 | 3.62 | 3.60 | 3.97/3.73 |
| 13C | 98.5 | 76.7 | 78.9 | 80.6 | 77.3 | 68.8 |
a Only observed in glycogen phospho-oligosaccharides.
b Only observed in amylopectin phospho-oligosaccharides.
c ND = not determined.