TABLE 2.

Phosphomonoester distribution in phospho-oligosaccharides from glycogen and amylopectin based on NMR analyses

The relative proportions of C2, C3, and C6 phosphorylation were estimated by integrating the corresponding signals in NMR experiments such as shown in Fig. 4. Samples were oligosaccharides prepared from the indicated source as described under “Experimental Procedures.” In the case of the glycogen TCA samples, the data refer to reevaluation of a previous sample (Ref. 26) as well as a new sample analyzed in the present study.

Sample	C2 phosphate	C3 phosphate	C6 phosphate
	%	%	%
Glycogen
TCA (2010)	28	53	19
TCA (2013)	30	39	31
KOH	29	37	34
Amylopectin	0	13	87