TABLE 3.
Determination of total phosphate and glucose-6-P in glycogen and amylopectin
Sourcea | Glycogen phosphate (mol/mol) × 10−3 |
% C6 phosphate | Glucose residues per |
Fold increase over WT |
|||
---|---|---|---|---|---|---|---|
Total | C6 | Total P | C6 P | Total P | C6 P | ||
WT (4) | 0.371 ± 0.03 | 0.063 ± 0.007 | 17 | 2700 | 15800 | 1 | 1 |
Epm2b−/− (5) | 1.612 ± 0.19 | 0.263 ± 0.015 | 16 | 620 | 3800 | 4.3 | 4.0 |
Epm2a−/− (4) | 2.785 ± 0.09 | 0.582 ± 0.021 | 21 | 360 | 1720 | 7.5 | 9.2 |
Rabbit (5–11) | 1.525 ± 0.10 | 0.324 ± 0.001 | 21 | 650 | 3090 | NAb | NA |
Amylo-pectin (4) | 4.403 ± 0.18 | 3.307 ± 0.034 | 76 | 227 | 300 | NA | NA |
a For the analyses of muscle glycogen from wild type (WT), Epm2b−/−, and Epm2a−/− mice, the numbers in parentheses refer to the number of samples, each from a separate mouse, that were analyzed. For the analyses of rabbit muscle glycogen and amylopectin, the numbers in parentheses refer to the number of independent, replicate analyses made from the same starting material.
b NA, not applicable.