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. 2014 Nov 21;290(2):841–850. doi: 10.1074/jbc.M114.607796

TABLE 3.

Determination of total phosphate and glucose-6-P in glycogen and amylopectin

Sourcea Glycogen phosphate (mol/mol) × 10−3
% C6 phosphate Glucose residues per
Fold increase over WT
Total C6 Total P C6 P Total P C6 P
WT (4) 0.371 ± 0.03 0.063 ± 0.007 17 2700 15800 1 1
Epm2b/ (5) 1.612 ± 0.19 0.263 ± 0.015 16 620 3800 4.3 4.0
Epm2a/ (4) 2.785 ± 0.09 0.582 ± 0.021 21 360 1720 7.5 9.2
Rabbit (5–11) 1.525 ± 0.10 0.324 ± 0.001 21 650 3090 NAb NA
Amylo-pectin (4) 4.403 ± 0.18 3.307 ± 0.034 76 227 300 NA NA

a For the analyses of muscle glycogen from wild type (WT), Epm2b−/−, and Epm2a−/− mice, the numbers in parentheses refer to the number of samples, each from a separate mouse, that were analyzed. For the analyses of rabbit muscle glycogen and amylopectin, the numbers in parentheses refer to the number of independent, replicate analyses made from the same starting material.

b NA, not applicable.

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