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. 2014 Dec 1;290(2):851–860. doi: 10.1074/jbc.M114.611509

FIGURE 2.

FIGURE 2.

UHRF1 interacts with TopoIIα. a, tandem affinity purification of SFB-tagged UHRF1 in ES cells was performed. Proteins identified by mass spectrometry are listed. b, co-immunoprecipitations (IP) between UHRF1 and TopoIIα in ES cells were performed using the antibodies indicated. Rabbit IgG was used as a control. c, MEF cells were stained with the antibodies indicated. d, 293T cells were transfected with SFB-tagged TopoI, TopoIIα, or TopoIIβ. Co-immunoprecipitations were performed using the antibodies indicated. IB, immunoblot.