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. 2014 Dec 1;290(2):851–860. doi: 10.1074/jbc.M114.611509

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FIGURE 3. — UHRF1 interacts with TopoIIα through its Tudor and PHD domains. a, the domain structures of WT UHRF1 and each deletion mutant are shown. b, HA-tagged WT and deletion mutants of UHRF1 were cotransfected with SFB-TopoIIα into 293T cells. Co-immunoprecipitations (IP) were performed using the antibodies indicated. c, HA-tagged WT and point mutants of UHRF1 were cotransfected with SFB-TopoIIα into 293T cells. Co-immunoprecipitations were performed using the antibodies indicated. d, endogenous TopoIIα in 293T cells was pulled down using recombinant proteins of GST-tagged domains of UHRF1 as indicated and detected. UBL, ubiquitin-like domain; IB, immunoblot.