FIGURE 4.

TopoIIα interacts with UHRF1 using two domains. a, the domain structures of WT TopoIIα and each deletion mutant are shown. Nuclear localization sequences (NLS) were added to the D5 mutant because it has a deletion of the original nuclear localization sequence in TopoIIα. b, SFB-tagged WT and deletion mutants of TopoIIα were transfected into 293T cells. Co-immunoprecipitations (IP) were performed using the antibodies indicated. c, SFB-tagged WT and deletion mutants of TopoIIα were transfected into 293T cells. Pulldown assay was performed using recombinant proteins of GST-tagged Tudor and PHD domains of UHRF1. IB, immunoblot; CBB, Coomassie Brilliant Blue.