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. 2014 Dec 1;290(2):851–860. doi: 10.1074/jbc.M114.611509

FIGURE 5.

FIGURE 5.

UHRF1 regulates the localization of TopoIIα at PCH. a, SFB-tagged WT and point mutants of UHRF1 were electroporated into MEF cells, and their localizations were visualized by staining with anti-FLAG antibody. b, SFB-tagged WT and deletion mutants of TopoIIα were electroporated into MEF cells, and their localizations were visualized by staining with anti-FLAG antibody. c, the localizations of endogenous TopoIIα in the different ES cells indicated were visualized by staining with anti-TopoIIα antibody. Twenty-five cells were quantified for each experiment in a and b, and 100 cells were quantified for each experiment in c. Error bars represent the mean ± S.D. KO, knock-out.