UHRF1 does not regulate the localization of TopoIIα through H3K9me3 binding.
a–d, the localizations of H3K9me3, TopoIIα, UHRF1, DNMT1, and 5-methylcytosine (5mC) in wild-type and Suv39h1/Suv39h2 double-negative (dn) MEF cells were visualized by staining with the antibodies indicated. The percentage of cells with PCH-staining foci (foci-positive cells) from three independent experiments is summarized. One-hundred cells were quantified for each experiment. Error bars represent the mean ± S.D.