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. 2014 Nov 5;290(2):861–871. doi: 10.1074/jbc.M114.586560

FIGURE 1.

FIGURE 1.

IL-17RD is up-regulated in RTECs. A, schematic of an epitope of a monoclonal antibody against IL-17RD (3F12). ICD, intracellular domain; TM, transmembrane. B–D, Western blot (IB) assays for validating the anti-IL-17RD antibody. HKE293T cells transfected with the indicated plasmids or shRNAs against IL-17RD (named shIL-17RDi-1 and shIL-17RDi-2) and tissue lysates from rat, mouse, and human were collected, and IL-17RD was examined by Western blot using the anti-IL-17RD antibody (3F12). Di, dimer; Mo, monomer. E, IL-17RD was up-regulated in RTECs of nephritis rats. Immunohistochemical staining for IL-17RD in sections from kidneys of IgA nephropathy and a control and 5/6 nephrectomized rats and sham controls are shown (bars, 200 μm). F, quantification of the IL-17RD expression in RTECs. Scores of IL-17RD positive cells in RTECs were calculated, and averaged scores were used to present the IL-17RD levels. p < 0.05 (*) and p < 0.001 (***).