FIGURE 8.

A model for ATP-dependent translation inhibition of PINK1. A and B, treatment with CHX blocks CCCP-induced Parkin mitochondrial translocation. HeLa cells expressing Venus-Parkin-WT and RFP-smac-mts were left untreated or were treated with 10 μg/ml CHX for 1.5 h and incubated with 20 μm CCCP. Parkin mitochondrial localization was visualized by fluorescent microscopy (A) and quantified with respect to increasing CHX concentration (B). C, proposed model for ATP-dependent translation inhibition of PINK1 as a regulatory mechanism that suppresses Parkin mitochondrial translocation.