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. 2014 Nov 12;290(2):926–940. doi: 10.1074/jbc.M114.621409

FIGURE 3.

FIGURE 3.

Direct post-transcriptional regulation of ERBB4 expression by miR-193a-3p. A, quantitative RT-PCR analysis of the miR-193a-3p levels in A549, HCC827, and H1975 cells treated with pre-miR-control, pre-miR-193a-3p, anti-miR-control, or anti-miR-193a-3p. B and C, Western blot analysis of ERBB4 protein levels in A549, HCC827, and H1975 cells treated with pre-miR-control, pre-miR-193a-3p, anti-miR-control, or anti-miR-193a-3p. B, representative image; C, quantitative analysis. D, quantitative RT-PCR analysis of ERBB4 mRNA levels in A549, HCC827, and H1975 cells treated with pre-miR-control, pre-miR-193a-3p, anti-miR-control, or anti-miR-193a-3p. E, firefly luciferase reporters containing wild-type (WT) or mutant (MUT) miR-193a-3p binding sites in the ERBB4 3′-UTR were co-transfected into A549, HCC827, and H1975 cells along with pre-miR-control, pre-miR-193a-3p, anti-miR-control, or anti-miR-193a-3p. Twenty-four hours post-transfection, the cells were assayed using a luciferase assay kit. ***, p < 0.001. Error bars, S.E.