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. 2014 Nov 12;290(2):926–940. doi: 10.1074/jbc.M114.621409

FIGURE 4.

FIGURE 4.

The role of miR-193a-3p targeting of ERBB4 in the regulation of proliferation, invasion, and apoptosis in lung cancer cells. A, the MTT viability assay was performed 12, 24, 36, and 48 h after the transfection of A549 cells with pre-miR-control, pre-miR-193a-3p, anti-miR-control, or anti-miR-193a-3p. B, the MTT viability assay was performed 12, 24, 36, and 48 h after the transfection of A549 cells with pre-miR-control plus control vector, pre-miR-193a-3p plus control vector, pre-miR-control plus ERBB4 vector, or pre-miR-193a-3p plus ERBB4 vector. C and D, Transwell analysis of invaded A549 cells treated with equal doses of pre-miR-control, pre-miR-193a-3p, anti-miR-control, anti-miR-193a-3p, pre-miR-control plus control vector, pre-miR-193a-3p plus control vector, pre-miR-control plus ERBB4 vector, or pre-miR-193a-3p plus ERBB4 vector. C, representative image; D, quantitative analysis. E and F, A549 cells were transfected with equal doses of pre-miR-control, pre-miR-193a-3p, anti-miR-control, anti-miR-193a-3p, pre-miR-control plus control vector, pre-miR-193a-3p plus control vector, pre-miR-control plus ERBB4 vector, or pre-miR-193a-3p plus ERBB4 vector. Cell apoptosis profiles were analyzed by flow cytometry. The biparametric histogram shows cells in early (bottom right quadrant) and late apoptotic states (top right quadrant). Viable cells are double negative (bottom left quadrant). E, representative image; F, quantitative analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, S.E.