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. 2014 Nov 12;290(2):926–940. doi: 10.1074/jbc.M114.621409

FIGURE 5.

FIGURE 5.

Down-regulation of ERBB4 by siRNA and up-regulation of ERBB4 by an overexpression vector in lung cancer cells. A and B, Western blotting analysis of ERBB4 protein levels in A549 cells treated with control siRNA, ERBB4 siRNA, control vector, or ERBB4 vector. A, representative image; B, quantitative analysis. C, quantitative RT-PCR analysis of ERBB4 mRNA levels in A549 cells treated with control siRNA, ERBB4 siRNA, control vector, or ERBB4 vector. D, the MTT viability assay was performed 12, 24, 36, and 48 h after the transfection of A549 cells with control siRNA, ERBB4 siRNA, control vector, or ERBB4 vector. E and F, Transwell analysis of invaded A549 cells treated with equal doses of control siRNA, ERBB4 siRNA, control vector, or ERBB4 vector. E, representative image; F, quantitative analysis. G and H, A549 cells were transfected with equal doses of control siRNA, ERBB4 siRNA, control vector, or ERBB4 vector. Cell apoptosis profiles were analyzed by flow cytometry. The biparametric histogram shows cells in early (bottom right quadrant) and late apoptotic states (top right quadrant). Viable cells are double negative (bottom left quadrant). G, representative image; H, quantitative analysis. ***, p < 0.001. Error bars, S.E.