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. 2014 Nov 12;290(2):926–940. doi: 10.1074/jbc.M114.621409

FIGURE 6.

FIGURE 6.

Effects of miR-193a-3p and ERBB4 on cell proliferation, invasion, and apoptosis in A549-Luc, A549-Luc-miR-193a-3p, and A549-Luc-ERBB4 cells. A, quantitative RT-PCR analysis of the miR-193a-3p levels in A549-Luc, A549-Luc-miR-193a-3p, and A549-Luc-ERBB4 cells. B, quantitative RT-PCR analysis of ERBB4 mRNA levels in A549-Luc, A549-Luc-miR-193a-3p, and A549-Luc-ERBB4 cells. C and D, Western blotting analysis of protein levels in A549-Luc, A549-Luc-miR-193a-3p, and A549-Luc-ERBB4 cells. C, representative image; D, quantitative analysis. E, the MTT viability assay was performed after 12, 24, 36, and 48 h in A549-Luc, A549-Luc-miR-193a-3p, and A549-Luc-ERBB4 cells. F and G, Transwell analysis of invaded A549-Luc, ERBB4 A549-Luc-miR-193a-3p, and A549-Luc-ERBB4 cells. F, representative image; G, quantitative analysis. H and I, cell apoptosis profiles of A549-Luc, A549-Luc-miR-193a-3p, and A549-Luc-ERBB4 cells by flow cytometry. The biparametric histogram shows cells in early (bottom right quadrant) and late apoptotic states (top right quadrant). Viable cells are double negative (bottom left quadrant). H, representative image; I, quantitative analysis. **, p < 0.01; ***, p < 0.001. Error bars, S.E.