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. 2014 Nov 17;290(2):941–949. doi: 10.1074/jbc.M114.608455

FIGURE 5.

FIGURE 5.

Shed syndecan-1 shuttles HGF to the nucleus and syndecan-1·HGF exists as a complex within the nucleus. A, conditioned medium containing high levels of sSDC1 and HGF was collected from myeloma cells and pretreated with hep III enzyme or added directly to CHO-pgsA745 cells. Nuclear lysates were collected, and an ELISA was performed to determine levels of HGF in the nucleus. *, p < 0.05 versus untreated controls. B, nuclear lysates were prepared from CHO-pgsA745 cells that had been incubated for 1 h in medium containing high levels of shed syndecan-1 and HGF. The level of HGF was determined with or without prior immunoprecipitation (IP) of syndecan-1 from the lysate.