FIGURE 1.

Bioinformatic analysis. A, expression of Bmdimm in silkworm tissues on day 3 of fifth larval instar based on microarray database information. B, location of B. mori dimm in the silkworm genome (left), gene structure (exon-intron diagram), and nucleotide and deduced amino acid sequences. The boxes are amino acid sequences of bHLH. C, bHLH motif in Bmdimm compared with other bHLH proteins, including the Mist subfamily of vertebrates, Dmdimm, and Rpdimm of invertebrates. Dots, identical amino acids; dashes, gaps introduced to maximize alignment. D, phylogenetic tree of bHLH transcription factors. The MEGA5 program was used with a neighbor-joining algorithm. GenBank accession numbers are listed in Table 1.