FIGURE 4.

Bmdimm protein interacts with Bmsage. A, interaction of Bmsage with Bmdimm. Panel a1, purified Bmdimm-GST used as prey protein was incubated with purified Bmsage-GST as bait protein and detected by anti-Bmsage. Panel a2, purified Bmsage-GST used as prey protein was incubated with Bmdimm-GST as bait protein and detected by anti-Bmdimm. Purified GST and BSA proteins were used as negative controls. B, binding of Bmdimm and Bmsage detected by ELISA. BSA was used as a negative control. Results are expressed as means ± S.D. of three independent experiments; *, p < 0.05. C, immunostaining of Bmdimm and Bmsage in BmE cells. Scale bar, 5 μm. D, nuclear extracts immunoprecipitated (IP) with anti-Bmsage or anti-Bmdimm followed by Western blot (WB) using Myc antibody (panel d1) or Bmsage antibody (panel d2).