FIGURE 5.

Bmdimm responds to JHA in silk glands in vivo and in vitro. A and C, expression of Bmdimm and fib-H in larvae in vivo after JHA induction assayed by qRT-PCR. JHA was dissolved in acetone, diluted to 1 μg/μl, and topically applied to larvae along the dorsal surface at 1 μg per g of fresh body weight. Acetone was used for the control. B and D, expression of Bmdimm and fib-H in silk glands in vitro after JHA induction assayed by qRT-PCR. Silk glands were dissected from the 1st day (V0) of the fifth larval instar and incubated with JHA for 0, 3, 6, 12, or 24 h. Results are expressed as means ± S.D. of three independent experiments. BmRpl3 expression is shown as a control.