Expression of JH receptor and BmKr-h1 in silk glands in vivo, in vitro, and in cultured B. mori cells.
A–C, BmMet1 (A), BmMet2 (B), and BmSRC (C) transcript levels determined by qRT-PCR in normally developing silkworms without JHA treatment. D, expression of BmKr-h1 in silk glands from silkworms treated with JHA or acetone (control). E, expression of BmKr-h1 in silk glands cultured in vitro. BmRpl3 expression was used as a control. Results are expressed as means ± S.D. of three independent experiments. F, expression of BmMet1, BmMet2, BmSRC, and BmKr-h1 in BmE and BmN cells analyzed by RT-PCR. BmRpl3 expression was used as a control.