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. 2014 Nov 4;290(2):972–986. doi: 10.1074/jbc.M114.606921

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FIGURE 8. — JH effect on induction of Bmdimm is mediated by the JH-Met-Kr-h1 signaling pathway. A and B, expression of BmKr-h1 after overexpression of BmMet2 assayed by qRT-PCR. C–E, expression of BmMet2 and Bmdimm after overexpression of BmKr-h1 assayed by qRT-PCR. F and G, expression of Brc-Z2 and Brc-Z4 after overexpression of BmKr-h1 assayed by qRT-PCR. BmE cells were transfected with 1 μg of 1180-A4/BmKr-h1 or 1180-A4/BmMet2 and incubated in medium containing 10 μm JHA or DMSO for 12 h. BmRpl3 expression is shown as a control. Results are expressed as means ± S.D. of three independent experiments; *, p < 0.05; **, p < 0.01. H, sequence of the Bmdimm promoter. Potential cis-elements were predicted using the MATINSPECTOR program. I, ChIP assays analyzing BmKr-h1 binding to the Kr response element in the promoter region of Bmdimm. Anti-HA and normal rabbit IgG were used for immunoprecipitation. Enrichment of promoter binding was analyzed by qRT-PCR in triplicate and expressed as a percentage over input. Quantified results are presented as means ± S.D.; *, p < 0.05. NS, no significance.