TABLE 2.
Primer sequences used in this study
F indicates forward, and R indicates reverse.
| Assay | Gene | Primer sequences (5′ to 3′) |
|---|---|---|
| RT-PCR | fib-H | F, ATACGCTTGGTCGTCAAAATCTG |
| R, TCTGTGTCATCTGCTTCATCTCG | ||
| RT-PCR | Bmdimm | F, ATGCCACACTGGGTAAC |
| R, GAAGAAACCTTGCGTCCG | ||
| RT-PCR | BmMet1 | F, AATCTTGCCACCAACAGC |
| R, ACCCAACGCACATCTTCT | ||
| RT-PCR | BmMet2 | F, ACGGCCATTAAATCCTTG |
| R, GGTAAACCCTCACGACAC | ||
| RT-PCR | BmSRC | F, TCAAACGAGTCAAATAGGGTCA |
| R, GCGGTCGGTGGTAGGGTT | ||
| RT-PCR | BmKr-h1 | F, TCACAACCTACGCCAACA |
| R, CGCTCCTCGTCACCTATC | ||
| RT-PCR | BmRpl3 | F, TCGTCATCGTGGTAAGGTCAA |
| R, TTTGTATCCTTTGCCCTTGGT | ||
| qRT-PCR | Bmsage | F, AGCAATCACGAAGGTCCGC |
| R, CGTATCGTGGTTGGAGTCGT | ||
| qRT-PCR | Bmdimm | F, CGTGGAACCCGCATTTGTA |
| R, AACCTCGGCAATCCAGTCG | ||
| qRT-PCR | fib-H | F, TATCCAGGACGAAGTAAGAAACAA |
| R, TCTGTGTCATCTGCTTCATCTCG | ||
| qRT-PCR | BmMet1 | F, AATCTTGCCACCAACAGC |
| R, ACCCAACGCACATCTTCT | ||
| qRT-PCR | BmMet2 | F, ACGGCCATTAAATCCTTG |
| R, GGTAAACCCTCACGACAC | ||
| qRT-PCR | BmMet2-ORF | F, ATGAAGCGTCCAAATAAC |
| R, TGACATCGTCCAAGAAAC | ||
| qRT-PCR | BmSRC | F, TCAAACGAGTCAAATAGGGTCA |
| R, GCGGTCGGTGGTAGGGTT | ||
| qRT-PCR | BmKr-h1 | F, AACCCATACTGGCGAGCG |
| R, ATACGACGGTGTACTTGC | ||
| qRT-PCR | BmKr-h1-ORF | F, ATGATAGGTGACGAGGAGC |
| R, CCAGTATGGGTTCGGTAG | ||
| qRT-PCR | SGF1 | F, CCTTTCTACAGACAAAACCAGC |
| R, GTCAGGATGTAGCGTCCAAAA | ||
| qRT-PCR | Brc-Z2 | F, TGGACAGTCAGACGCAACA |
| R, TAAGAACGGCGGACGAG | ||
| qRT-PCR | Brc-Z4 | F, TATGGCCCTTCCAACCCTGAT |
| R, AGGTGTTGCTGCTCCGTGTG | ||
| qRT-PCR | BmRpl3 | F, TTCGTACTGGCTCTTCTCGT |
| R, CAAAGTTGATAGCAATTCCCT | ||
| ChIP-qPCR | fibH-E-box | F, TGGACAGATTTGGCTTTG |
| R, CACTAGAGGAACGGGACA | ||
| ChIP-qPCR | Bmdimm-Kr CRE | F, GTCAGTCCTACGGCATAT |
| R, GATAGTGCCACAACATAAAA |