TABLE 3.
Primers for DNA constructs in this study
| Plasmid name | PCR template | Primer sequences (5′ to 3′) |
|---|---|---|
| pET-28a/Bmdimm | cDNA | F, CGCGGATCCATGCGCCCAAGACGCGCT |
| R, CCCAAGCTTCAGAAGAAACCTTGCG | ||
| fib-H865-Luc | Genomic DNA | F, CGGGGTACCAAGCTTGTTGTACAAAACTG |
| R, CTAGCTAGCGCTGATTTGAAAAAGTTGAA | ||
| fib-H400-Luc | Genomic DNA | F, CGGGGTACCATTTACCCATCCAAGGCATTC |
| R, CTAGCTAGCGCTGATTTGAAAAAGTTGAA | ||
| fib-H37-Luc | Genomic DNA | F, CGGGGTACCATTTTCAGTATAAAAAG |
| R, CTAGCTAGCGCTGATTTGAAAAAGTTGAA | ||
| pGEX-4T-Bmdimm | cDNA | F, CGCGGATCCATGCCACACTGGGTAACT |
| R, ATTTGCGGCCGCTCAGAAGAAACCTTGCG | ||
| pGEX-4T-Bmdimm-Mut-E108T | pGEX-4T-Bmdimm | F, GTCTCGAGAGCAATACAAGAGAAAGAATGCGA |
| R, GCATTCTTTCTCTTGTATTGCTCTCGAGACGA | ||
| pGEX-4T-Bmdimm-Mut-R109T | pGEX-4T-Bmdimm | F, GTCTCGAGAGCAATGAAACAGAAAGAATGCGA |
| R, ATTCGCATTCTTTCTGTTTCATTGCTCTCGAG | ||
| pGEX-4T-Bmdimm-Mut-R113T | pGEX-4T-Bmdimm | F, AAAGAGAAAGAATGACAATGCATTCCCTCAAC |
| R, CGGTTGAGGGAATGCATTGTCATTCTTTCTCT | ||
| pGEM-T-BmKr-h1 | cDNA | F, ATGATAGGTGACGAGGAGCGAG |
| R, CTATGATTCTGTAGCTGGCG | ||
| 1180-BmKr-h1 | cDNA | F, CGCGGATCCATGTACCCATACGATGTTCCAGATTACGCTATAGGTGACGAGGAGCGAG |
| R, ATTTGCGGCCGCCTATGATTCTGTAGCTGGCG | ||
| 1180-Bmdimm | pGEX-4T-Bmdimm | F, CGCGGATCCATGGAACAAAAACTCATCTCAGAAGAGGATCTGCCACACTGGGTAACT |
| R, ATTTGCGGCCGCTCAGAAGAAACCTTGCG | ||
| 1180-Bmsage | pGEX-4T-Bmsage | F, CGCGGATCCATGGATTACAAGGATGACGACGATAAGTACAATCAAACATAC |
| R, ATTTGCGGCCGCTTAGTATCTCTGTTGACGC | ||
| 1180-BmMet2 | cDNA | F, CGCGGATCCATGGATTACAAGGATGACGACGATAAGGCTGATTGGTCTCTG |
| R, ATTTGCGGCCGCTTATTGTTGTTGGTTTTTCT |