Table 1.
Antibodies
| Antibody (Antigen) | Species | Catalogue | Supplier (Location) | Specificity (Dilution) |
|---|---|---|---|---|
| ChAT (human placental enzyme; NM_020549.3) | Goat | AB144P | Chemicon (Temecula, CA) | Firth et al. 2003 (1:200) |
| α3β2 Nicotinic AChRs (Bovine and mouse, recognizes an epitope AA 66–76 of the nicotinic AChR α1 subunit; also binds to native human α1, α3, and α5.) | Rat | MAb210 | Generous gift of Dr. Jon Lindstrom, University of Pennsylvania (Philadelphia, PA) | Keyser et al. 2000 (1:200) |
| α7 Nicotinic AChRs (Sequence wkfaacvvdrlclmafsvft maps to the cytoplasmic loop between the TM3 and -4 and partially into TM4 of the human α7 subunit.) | Goat | SC1447 | Santa Cruz Biotechnology (Santa Cruz, CA) | Dmitrieva et al. 2007 (1:200) |
| m1 Muscarinic AChR (GST fusion protein and part of i3 intercellular loop of human m1 muscarinic AChR; AA 227–353; accession P11229) | Rabbit | AB5164 | Chemicon | Levey et al. 1990; Dorje et al. 1991; Hersh et al. 1994; Strang et al. 2010 (1:50) |
| m2 Muscarinic AChR (clone M2-2-B3, i3 loop of m2 receptor; AA 225–359; fused to GST) | Rat | MAb367 | Chemicon | Levey et al. 1995; Strang et al. 2010 (1:100) |
| m3 Muscarinic AChR (peptide analogue of the carboxyl terminal of muscarinic m3 receptor covalently bonded to carrier protein) | Rabbit | AS-3741S | Research & Diagnostic Antibodies (Las Vegas, NV) | Ndoye et al. 1998 |
| WB-3741S | Webber Biotechnology (Las Vegas, NV) | Strang et al. 2010 (1:300) | ||
| m4 Muscarinic AChR (clone 18C7.2, m4 receptor i3 loop; human; fused to GST; NM_000741.2) | Mouse | MAb1578 | Chemicon | Wang et al. 2001 Strang et al. 2010 (1:200) |
| m5 Muscarinic AChR (synthetic peptide from the 3rd cytoplasmic domain of human muscarinic AChR m5; NM_012125.2) | Rabbit | AB9454 | Chemicon | Strang et al. 2010 (1:200) |
ChAT, choline acetyltransferase; AChR, ACh receptor; AA, amino acids; TM, transmembrane; GST, glutathione-S-transferase.