Figure 7.

Innate and adaptive immune response against Ad. (A) Assessment of innate immune response against naked DWP418, DWP418/ABP, or DWP418/PPSA. Serum samples from mice systemically administered 2 × 10¹⁰ VP of naked DWP418, DWP418/ABP, or DWP418/PPSA were collected 6 h post treatment. IL-6 levels were determined by ELISA. **P < 0.01 between PBS and naked DWP418 group. (B,C) Adaptive immune responses against naked Ad and Ad/PPSA. Naked Ad (dE1/GFP) or Ad/PPSA complex were incubated with and without Ad-specific neutralizing antibody-containing serum, and then GFP expression level was observed by fluorescence microscopy (B) and an FACScan flow cytometer (C). ***P < 0.001 between with and without the presence of neutralizing Ab for naked Ad.