Skip to main content
. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Nature. 2014 Nov 5;516(7531):391–394. doi: 10.1038/nature13819

Figure 1.

Figure 1

A relative positional shift of limbs, genitalia and the cloaca in squamates. μCT-scans of mouse (a), anole (b), python (c) and house snake (d) lumbo-sacral regions (highlighted in sketch in white) at embryonic stages, illustrating the position of the developing external genitalia. (e-h) 3D-reconstructions of cloacal volumes. The cloaca is located at the same anterior-posterior position as the limb in squamates (f-h), however, is positioned more posteriorly in the mouse (e). (i-l) Transversal sections stained for β-catenin and Shh, indicating the conservation of a cloacal signaling center in all four species. Scale bars, 200 μm. gt: genital tubercle; hp: hemipenis; lb: limb; cl: cloaca.