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. Author manuscript; available in PMC: 2016 Jan 7.
Published in final edited form as: Chemistry. 2014 Nov 7;21(2):867–874. doi: 10.1002/chem.201403479

Figure 1.

Figure 1

The design of a zinc(II)-sensitive, organelle-specific, intramolecular FRET-conjugate; L: coordinating solvent or counterion. Attributes of a “high-performance dye” include a large molar absorptivity and fluorescence quantum yield in physiological buffer solutions, high photostability, a narrow emission band, and compatibility with conventional excitation/emission filter sets.