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Localization of compound 4 (green) compared to that of a lysosome-associated membrane protein (LAMP) fused to the fluorescent protein FusionRed (red). HeLa (S3) cells were transiently transfected to express FusionRed-LAMP, then loaded with 4 (2 µm) immediately prior to live-cell multicolor SIM imaging (green: 495–550 nm; red: 570–620 nm).
