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. 2015 Jan 14;10(1):e0116766. doi: 10.1371/journal.pone.0116766

Figure 3. Buprenorphine temperature aversion in a dose-dependent and specific manner.

Figure 3

A, Buprenorphine (5μM) significantly reduced 24.5°C and 31.5°C aversion when compared to vehicle treated larvae (2-way anova post hoc comparison) and eliminated aversion to 24.5°C and 31.5°C as measured by no significant difference from chance (1-way anova against a hypothetical mean of 50%). Buprenorphine did not induce aberrant zone preference (1-way anova against a hypothetical mean of 50%) and did not differ from vehicle treated larvae in the 28.5°C vs 28.5°C assay (2-way anova post hoc comparison). B, Buprenorphine significantly reduced AITC sensitized preference for 24.5°C and aversion for 31.5°C when compared to vehicle treated larvae (2-way anova post hoc comparison). Buprenorphine did not induce aberrant zone preference (1-way anova against a hypothetical mean of 50%) and did not differ from vehicle treated larvae in the AITC sensitized 28.5°C vs assay (2-way anova post hoc comparison). C, Buprenorphine inhibits AITC sensitized thermal aversion in a dose-dependent manner. D, Buprenorphine inhibition of AITC sensitized thermal aversion is reversed by addition of naloxone (2-way anova post hoc comparisons). * p<0.05, ** p<0.01, *** p<0.001. Error bars represent SEM.