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. 2015 Jan 14;10(1):e0116766. doi: 10.1371/journal.pone.0116766

Figure 5. Analgesic molecules have differential effects on thermal aversion.

Figure 5

A, Clonidine (5μM) significantly reduced 24.5°C and 31.5°C aversion when compared to vehicle treated larvae (2-way anova post hoc comparison) and eliminated aversion to 24.5°C and 31.5°C as measured by no significant difference from chance (1-way anova against a hypothetical mean of 50%). Clonidine did not induce aberrant zone preference (1-way anova against a hypothetical mean of 50%) and clonidine treated larvae did not differ from vehicle treated larvae in the 28.5°C vs 28.5°C assay (2-way anova post hoc comparison). B, Clonidine significantly reduced AITC sensitized aversion to 31.5°C but not 24.5°C preference when compared to vehicle treated larvae (2-way anova post hoc comparison). Clonidine did not induce aberrant zone preference (1-way anova against a hypothetical mean of 50%) and did not differ from vehicle treated larvae in the AITC sensitized 28.5°C vs 28.5°C assay. C, HC-030031 (50μM) treated larvae did not differ from vehicle-treated larvae in avoidance of either 24.5°C or 31.5°C zones (p>0.05, p>0.05, respectively, 2-way anova post hoc comparison. D, HC-030031 significantly reduced AITC sensitized aversion to 24.5°C and 31.5°C when compared to vehicle treated larvae (p<0.0X, p<0.01, respectively, 2-way anova post hoc comparison). HC-030031 did not induce aberrant zone preference (p>0.05, 1-way anova against a hypothetical mean of 50%) and did not differ from vehicle treated larvae in the AITC sensitized 28.5°C vs 28.5°C assay. E, TC-N 1752 (5μM) treated larvae did not differ from vehicle-treated larvae in avoidance of either 24.5°C or 31.5°C zones. F, TC-N 1752 significantly reduced AITC sensitized aversion to 24.5°C and 31.5°C when compared to vehicle treated larvae. TC-N 1752 did not induce aberrant zone preference (p>0.05, 1-way anova against a hypothetical mean of 50%) and did not differ from vehicle treated larvae in the AITC sensitized 28.5°C vs 28.5°C assay. G, Amitriptyline (0.5μM) treated larvae did not differ from vehicle-treated larvae in avoidance of either 24.5°C or 31.5°C zones (p>0.05, p>0.05, respectively, 2-way anova post hoc comparison). H, Amitriptyline significantly reduced AITC sensitized aversion to 31.5°C but not 24.5°C when compared to vehicle treated larvae (p<0.05, p>0.05 respectively, 2-way anova post hoc comparison). Amitriptyline did not induce aberrant zone preference (p>0.05, 1-way anova against a hypothetical mean of 50%) and did not differ from vehicle treated larvae in the AITC sensitized 28.5°C vs 28.5°C assay (p>0.05, 2-way anova post hoc comparison). * p<0.05, ** p<0.01, *** p<0.001. Error bars represent SEM.