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. 2015 Jan 15;26(2):256–269. doi: 10.1091/mbc.E14-10-1451

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© 2015 Ren et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Art1 interaction with Rgf3. (A) GFP-Art1 was expressed or not in wild-type or Rgf3-FLAG cells. An anti-FLAG immunoblot of anti-FLAG (left) or anti-GFP (right) immunoprecipitations from the indicated strains. (B) The indicated strains were spotted in 10-fold serial dilutions on YE plates and incubated at the indicated temperatures. (C, D) Live-cell images of the indicated strains grown in YE at 25°C. Scale bars, 3 μm. (E) The indicated strains were spotted in 10-fold serial dilutions on YE plates containing 0.5 mg/ml calcofluor or 1 M KCl and incubated at 29°C.