Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 15;26(2):256–269. doi: 10.1091/mbc.E14-10-1451

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Ren et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 9: — Pos1 interaction with Spa2. (A) An anti-FLAG immunoblot (top) and an anti-GFP immunoblot (bottom) of anti-FLAG (left) or anti-GFP (right) immunoprecipitations from the indicated strains. (B) Representative live-cell images of the indicated strains grown at 19°C. Scale bars, 3 μm. Bottom is merged with differential interference contrast image. (C) Representative bright-field live-cell images of the indicated strains. Scale bar, 3 μm. The indicated phenotypes of these strains grown at 19°C are scored on 300 cells and shown in the graphs below the images. (D) Invasive growth assays for strains of the indicated genotypes on 2% agar. Cells were spotted on rich medium and incubated for 20 d at 29°C (top). Colonies were then rinsed under a stream of water and rubbed off (bottom). (E) crn1-GFP pos1∆ cells overexpressing spa2⁺ were imaged live at 25°C. Scale bar, 5 μm.