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. 2015 Jan 15;26(2):316–326. doi: 10.1091/mbc.E14-01-0019

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© 2015 Selvi, Swaminathan, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — CARM1 is involved in sensory response. (A) Gene expression analysis of NF1 and Sox1 in CARM1 morphants in comparison with control (n = 3; t-test p values: NF1, 0.0293; Sox1, 0.0536. (B) Assay to measure response to stimulus. About 200 embryos injected with control/CARM1 morpholino at single-cell stage and 200 embryos each treated with DMSO/TBBD at 10 h postfertilization were observed for response to stimulus (100 embryos each were analyzed on 3, 4, and 5 d postfertilization [dpf]). (C) Comparison of the response to stimulus between DMSO-treated embryos and embryos treated with increasing concentrations of TBBD on 3, 4, and 5 dpf (n = 100; *p < 0.05, **p < 0.01, ***p < 0.005). The percentage of nonresponders showed a drastic increase at higher concentrations of inhibitor, especially for the 4- and 5-dpf treatments. (D) Comparison of the response to stimulus between control and CARM1 morphants on 3, 4, and 5 dpf (n = 100; p values: day 3, 0.0113; day 4, 0.007; day 5, 0.0378). The effect on the sensory response is more pronounced in the 3-dpf samples and is diluted out over the developmental stage. (E) Rescue of sensory response upon injection of in vitro–transcribed CARM1 RNA. The loss in sensory response observed in CARM1 morphants was rescued to a greater extent on 3 dpf. The effect of the morpholino and the RNA decreased on 4 and 5 dpf owing to dilution with time.