FIGURE 4:

Bax-permeabilized OMVs shifted gradually toward lower MFI. OMVs loaded with 10-, 70-, or 500-kDa F-dex were treated with 0, 5, 10, or 20 nM Bax and 45 nM cBid and analyzed by flow cytometry every 5 min for 45 min. For clarity, histograms at every 10 min are shown. Data are representative of three independent experiments. Note that there were no discrete peaks at the MFI of the empty OMV peak (in solid gray) as shown in the control experiment for each dextran size (bottom panels). Rather, empty OMVs comprised only a tail to the left of the main peak; the main OMV peak gradually shifted toward lower MFI.