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. Author manuscript; available in PMC: 2015 Jan 14.
Published in final edited form as: Fungal Genet Biol. 2013 Apr 19;60:64–73. doi: 10.1016/j.fgb.2013.04.005

Fig. 4.

Fig. 4

Treatment of C. neoformans with WGA affects polysaccharide secretion, but not cell viability or serologic properties of GXM. (A) Incubation of cryptococci with WGA followed by GXM determination in supernatants revealed a dose-dependent inhibition of polysaccharide extracellular release. (B) Immunofluorescence microscopy demonstrating that exposure of C. neoformans to WGA did not affect the recognition of GXM by a monoclonal antibody raised to the polysaccharide. Analysis of fungal proliferation by CFU counting (C) or culture spectrophotometry (D) revealed that growth rates were similar in control systems (CT, incubated in the absence of the lectin) or in cultures that were supplemented with WGA.