Treatment of C. neoformans with WGA affects the transcript levels of genes required for capsule formation. The relative expression of β-tubulin (TUB), phosphomannose isomerase 1 (MAN1), α-1,3-mannosyltransferase (CMT1), Golgi reassembly and stacking protein (GRASP), capsule-related (CAP) proteins 59, 10, 64 and 60, G-protein alpha subunit 1 (GPA1) and cyclic AMP (cAMP)-dependent protein kinase catalytic subunit 1 (PKA1) was quantified by qRT-PCR. RNA amounts in each sample were normalized using the threshold cycle (CT) values obtained for the actin gene.