Table 1.
List of primers used in qRT-PCR.
| Gene | Accession numbera | Primer | Sequence (5′–3′) |
|---|---|---|---|
| CAP59 | CNAG_00721 | CnCAP59F | AACCCGAACGAAGAAACCTC |
| CnCAP59R | ACCCCAGCACCACACATACTC | ||
| CAP64 | CNAG_02885 | CnCAP64F | ACAAGGAAAGGGCATTCAGAG |
| CnCAP64R | TATCACTGGGACGGTCAGAAAG | ||
| CAP10 | CNAG_07554 | CnCAP10F | TCTTCTTCCGTCATTCGTTC |
| CnCAP10R | TTCTCCCGTACCTCTTCTTG | ||
| PKA1 | CNAG_00396 | CnPKA1F | CTGAACGAGAGATGTTGGTAC |
| CnPKA1R | GCTGACTTTTACGAAGAAGG | ||
| MAN1 | CNAG_04312 | CnMAN1F | TTGATGAGAAGCCTGATGTC |
| CnMAN1R | TAGGGATTCGGTGAGAGAAG | ||
| GPA1 | CNAG_04505 | CnGPA1F | GATGAGGCTGATGTTCTGAG |
| CnGPA1R | CTTCTTTCTCTCGCTTCTCTG | ||
| CMT1 | CNAG_03158 | CnCMT1F | CTCGTACAGCCTATTCCAAC |
| CnCMT1R | GACATCACCTCTCCTGAAAC | ||
| GRASP | CNAG_03291 | CnGRASPF | GAGGGAGAGGGAATAGTAGTG |
| CnGRASPR | CTTTTGACGCTTCTTCTGAC | ||
| CAP60 | CNAG_00600 | CnCAP60F | CGCATATTACCCCCTTGAAG |
| CnCAP60R | CGGCATTGTTGAGTTGACTG | ||
| ACT1 | CNAG_00483 | CnACTF | CCTTGCTCCTTCTTCTAT |
| CnACTR | CTCGTCGTATTCGCTCTT | ||
| TUB | CNAG_01840 | CnTUBF | TGTCCTCTACCTTCATTTGC |
| CnTUBR | CCATACCCTCTCCAGTGTAC |
a
Cryptococcus neoformans H99 Genome Database at Broad Institute – http://www.broadinstitute.org/annotation/genome/cryptococcus_neoformans/MultiHome.html. Gene products are phosphomannose isomerase 1 (MAN1), a-1,3-mannosyltransferase (CMT1), Golgi reassembly and stacking protein (GRASP), capsule-related (CAP) proteins 59, 10, 64 and 60, G-protein alpha subunit 1 (GPA1), cyclic AMP (cAMP)-dependent protein kinase catalytic subunit 1 (PKA1) and actin (ACT1).